Learn More About The P53 Antibody For Research

Jan 16, 18 Learn More About The P53 Antibody For Research

The P53 antibody is a tumor suppressant gene that is expressed in many tissue types. It is also involved in the regulation of cell growth, apoptosis, and replication. It is designed to bind with mdm2, the human papilloma virus E6 protein, and the SV40 T antigen. It can sense DNA damage and can sometimes help with repairs. A mutation involving these antibodies can be found in many malignant tumors, such as ovarian, melanoma, bladder, lung, breast, and colon.

More Information

The P53 antibody is intended to be used for research and not diagnostic procedures. It does have a clone called SP5, and the immunogen is the recombinant human protein of the same name (full length, wild type). The isotype is the Rabbit IgG, and it has an undetermined epitope. The molecular weight of this is 53kDa.

Applications

This antibody is designed for Immunohistochemistry (IHC) applications. To start, you will need to prepare the specimen using a paraffin-embedded or Formalin-fixed tissue sample. Deparaffinized slides are also essential and can be achieved with xylene, its alternatives, or graded alcohols.

You’ll find a pre-diluted and concentrate formula available for purchase. The pre-diluted formulation makes it easier because you don’t have another step. However, you can purchase the concentrated version and dilute the antibody using a ratio of 1:100. These are estimations, so you may need to change the dilution rate.

To retrieve the antigen, you must boil the tissue sections using a Citrate buffer with a pH of 6.0. This should be boiled for ten minutes and then cooled for 20 until it reaches room temperature. It must also be incubated for 30 minutes while at room temperature.

The positive control is the colon carcinoma with cellular localization occurring in the nucleus.

The P53 antibody can be used to research tumors and other mutations. Visit Spring Bioscience at http://www.springbio.com to learn more.

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