How To Use The CD117 In Research

Sep 29, 17 How To Use The CD117 In Research

The CD117 antibody doesn’t interfere with binding the SCF to the c-kit. It can precipitate both occupied and unoccupied forms of the c-Kit. The binding of SCF to the c-kit-encoded receptor Tyrosine Kinase-III stimulates many biochemical responses that can culminate in the proliferation, survival, and migration of cells. It is essential for melanogenesis, gametogenesis, and hematopoiesis.

More Information

The CD117 antibody has no clone, and the immunogen is the synthetic peptide in the cytoplasmic domain of the human c-kit protein. The isotype is the Rabbit IgG, and it has an undetermined epitope. The molecular weight of this antibody is 145kDa.

Applications

You can use Immunohistochemistry applications to research this antibody. You will need to use paraffin-embedded or Formalin-fixed tissues. Xylene, graded alcohols, or a xylene alternative are essential for deparaffinized slides.

If you purchased the concentrated format of this product, you will need to dilute the antibody using a ratio of 1:100. However, your methods or protocols may require different dilution options, so pay close attention to your work. If you need the normal ratio, you can also find a pre-diluted formula available for purchase.

To retrieve the antigen, you should boil the tissue section in a 10mM Citrate buffer with a pH of 6.0. Do this for ten to 20 minutes, depending on the protocols and allow the concoction to cool to room temperature for no more than 20 minutes.

Once cooled the appropriate amount of time, you should incubate at room temperature for ten more minutes.

Slides should be washed with a PBS/0.05% Tween solution between steps.

The positive control is the skin, tonsil, or gastrointestinal stromal tumor with cellular localization occurring in the Cytoplasm.

The CD117 can be used for research a variety of ways. Visit Spring Bioscience to learn more about purchasing options.

3 people like this post.

Be Sociable, Share!
    Shares
    Share This