Learn More About The P53 Antibody For Research

Jan 16, 18 Learn More About The P53 Antibody For Research

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The P53 antibody is a tumor suppressant gene that is expressed in many tissue types. It is also involved in the regulation of cell growth, apoptosis, and replication. It is designed to bind with mdm2, the human papilloma virus E6 protein, and the SV40 T antigen. It can sense DNA damage and can sometimes help with repairs. A mutation involving these antibodies can be found in many malignant tumors, such as ovarian, melanoma, bladder, lung, breast, and colon. More Information The P53 antibody is intended to be used for research and not diagnostic procedures. It does have a clone called SP5, and the immunogen is the recombinant human protein of the same name (full length, wild type). The isotype is the Rabbit IgG, and it has an undetermined epitope. The molecular weight of this is 53kDa. Applications This antibody is designed for Immunohistochemistry (IHC) applications. To start, you will need to prepare the specimen using a paraffin-embedded or Formalin-fixed tissue sample. Deparaffinized slides are also essential and can be achieved with xylene, its alternatives, or graded alcohols. You’ll find a pre-diluted and concentrate formula available for purchase. The pre-diluted formulation makes it easier because you don’t have another step. However, you can purchase the concentrated version and dilute the antibody using a ratio of 1:100. These are estimations, so you may need to change the dilution rate. To retrieve the antigen, you must boil the tissue sections using a Citrate buffer with a pH of 6.0. This should be boiled for ten minutes and then cooled for 20 until it reaches room temperature. It must also be incubated for 30 minutes while at room temperature. The positive control is the colon carcinoma with cellular localization occurring in the nucleus. The P53 antibody can be used to research tumors and other mutations. Visit Spring Bioscience at http://www.springbio.com to learn more. 1 person likes this...

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How To Use The CD117 In Research

Sep 29, 17 How To Use The CD117 In Research

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The CD117 antibody doesn’t interfere with binding the SCF to the c-kit. It can precipitate both occupied and unoccupied forms of the c-Kit. The binding of SCF to the c-kit-encoded receptor Tyrosine Kinase-III stimulates many biochemical responses that can culminate in the proliferation, survival, and migration of cells. It is essential for melanogenesis, gametogenesis, and hematopoiesis. More Information The CD117 antibody has no clone, and the immunogen is the synthetic peptide in the cytoplasmic domain of the human c-kit protein. The isotype is the Rabbit IgG, and it has an undetermined epitope. The molecular weight of this antibody is 145kDa. Applications You can use Immunohistochemistry applications to research this antibody. You will need to use paraffin-embedded or Formalin-fixed tissues. Xylene, graded alcohols, or a xylene alternative are essential for deparaffinized slides. If you purchased the concentrated format of this product, you will need to dilute the antibody using a ratio of 1:100. However, your methods or protocols may require different dilution options, so pay close attention to your work. If you need the normal ratio, you can also find a pre-diluted formula available for purchase. To retrieve the antigen, you should boil the tissue section in a 10mM Citrate buffer with a pH of 6.0. Do this for ten to 20 minutes, depending on the protocols and allow the concoction to cool to room temperature for no more than 20 minutes. Once cooled the appropriate amount of time, you should incubate at room temperature for ten more minutes. Slides should be washed with a PBS/0.05% Tween solution between steps. The positive control is the skin, tonsil, or gastrointestinal stromal tumor with cellular localization occurring in the Cytoplasm. The CD117 can be used for research a variety of ways. Visit Spring Bioscience to learn more about purchasing options. 3 people like this...

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MEP Engineers in Chattanooga TN Play Important Roles in Large Building Projects

Feb 09, 17 MEP Engineers in Chattanooga TN Play Important Roles in Large Building Projects

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Designing and constructing large commercial buildings often turns out to be even more complex and challenging than it might seem. For buildings meant to eventually house hundreds or thousands of occupants, a great deal of highly sophisticated planning must inevitably be done. While architects might sometimes grab a large share of the glory that follows with the distinctive visual designs they deliver, much of the work that goes on will normally remain more or less unseen. Focusing on all the mechanical, electrical, and plumbing details that are needed to make any large building functional, for example, MEP Engineers in Chattanooga TN do work that is arguably at least as important. As can be seen at caengrs.com, having this kind of expertise available is never something to be taken for granted. Many MEP Engineers in Chattanooga TN will have spent decades in the field building up the wide-ranging kind of expertise needed to succeed. When even relatively small systems of these kinds often turn out to be so complicated, the far more expansive ones that serve large commercial buildings can truly be daunting. As a result, the profession is never short on challenges, which turns out to be a source of great satisfaction for many. In addition to ensuring that building occupants or users will eventually be able to enjoy all the comfort and amenities they might hope for, engineers who focus on this kind of work deliver other benefits, as well. Large commercial buildings can consume many thousands of dollars worth of energy every month, and engineers who understand how to design and install more efficient supporting systems can often help keep these costs down. In fact, they can easily provide many millions of dollars worth of value over the lifetime of a given building, whether by planning an especially efficient set of systems for a new facility or upgrading those of an older one. While every large building project includes many different kinds of highly demanding work, some of these roles receive less...

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